In vivo assays

We can provide assays on primary and on immortalised cell lines, including signalling and functional assays. Some examples include:

GPCR signalling assays for compounds screening (cAMP, pERK)

Inflammatory readouts (human and mouse cell lines, whole blood assay, PBMCs, neutrophils, macrophages, etc)

Evaluation of markers by microscopy (biochemical staining, immunofluorescence)

Gene expression assays

DNA methylation analysis

Protein expression quantification

Lipid mediators

Cellular metabolism (mitochondrial biology, metabolic flux)

Apoptosis assays, DNA damage determination

Detection of senescence markers (SA-βGal, lipofuscin, IF)

Cell viability

ELISA, EIA assays (cell supernatants or lysates, plasma samples, etc)

Would healing assay

Endothelial cells assays, angiogenesis, VWF secretion assay

Chemotaxis assay

Macrophage phagocytosis

T cells assays

iPSC in vitro assays

Genotyping, SNP detection

Pharmacology and disease models

Disease areas

We provide a wide range of models and assays to support your drug development programs to evaluate efficacy of candidates in multiple areas:

Inflammation (acute, chronic, systemic)

Rheumatology (RA, OA)

Fibrosis (lung, liver, skin)

Cardiovascular (myocardial infarction, hypertension, atherosclerosis)

Gastrointestinal (colitis, colon cancer)

Nephrology (AKI, CKD, polycystic kidney disease)

Pain research

Diabetes

Obesity

Autoimmune diseases

Mitochondrial diseases

Metabolic diseases

Central nervous system

Angiogenesis

Knock-out models, diet-induced models

Testing and Evaluation

We offer tailored experimental design, with evaluation of the most appropriate endpoints and measurements to meet your specific needs.

Compound delivery through multiple routes

Scoring, disease monitoring

Histological analysis (FFPE, tissue staining, IF, whole-section scanning)

Analysis of plasma, serum, tissues lysates

Protein analysis, gene expression

Electrocardiography

Organ and whole-body imaging (CT, MRI, fluorescence, bioluminescence)

Sensory thresholds

Protein expression purification service

Production and purification in mammalian cells of recombinant proteins and particularly antibodies.

Biological samples

We can provide with biological samples obtained from pe-clinical models or from in vitro systems for your evaluation of your own markers.

Data analysis and bioinformatics

SNP genotyping - primer design, Sanger sequencing and analysis

Bioinformatics - differential gene expression analysis, functional analysis

OMICS data analysis (bulk and single-cell RNAseq, proteomics)

Statistical genetics

Sequencing association studies

Technical capabilities

Cellular biology

Molecular biology

Imaging services

Flow Cytometry

Extracellular vesicles research

NETs quantification

Gene expression (qPCR, OMICs)

SeaHorse metabolic analysis

In vitro and in vivo Pharmacology

Class-II cell culture of primary and immortalised cell lines

Neuronal health and sensitisation

Project design

With our flexible approach, we can advise and help you tailor our contract research services to your needs, helping you decide the right assay or model to address your scientific questions, the most relevant endpoints to measure, and propose specific methodologies or protocols.